A secure platform for developing and sharing reproducible methods.https://protocols.io/protocols.rssprotocols.ioen© 2026 protocols.io, part of Springer Nature. All rights reservedprotocols.io© 2026 protocols.io, part of Springer Nature. All rights reserved.info@protocols.iowww.protocols.io/view/human-whole-blood-pbmc-isolation-b2qqqdvwprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.kqdg3pez1l25/v1

Human Whole Blood PBMC Isolation]]>Human Whole Blood PBMC IsolationNicholas C Buitrago-Pocasangredx.doi.org/10.17504/protocols.io.kqdg3pez1l25/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.kqdg3pez1l25/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.kqdg3pez1l25/v1www.protocols.io/view/human-whole-blood-pbmc-isolation-b2qqqdvwwww.protocols.io/view/human-csf-processing-for-10x-scrna-seq-b6tbreinprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.eq2lyn63rvx9/v1

Human CSF Processing for 10x scRNA-seq]]>Human CSF Processing for 10x scRNA-seqNicholas C Buitrago-Pocasangredx.doi.org/10.17504/protocols.io.eq2lyn63rvx9/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.eq2lyn63rvx9/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.eq2lyn63rvx9/v1www.protocols.io/view/human-csf-processing-for-10x-scrna-seq-b6tbreinwww.protocols.io/view/manual-dna-extraction-protocol-for-campylobacter-d-b88irzueprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.36wgq7b6kvk5/v1

Manual DNA Extraction Protocol for Campylobacter Detection in Canine and Bovine Feces ]]>Manual DNA Extraction Protocol for Campylobacter Detection in Canine and Bovine Feces dx.doi.org/10.17504/protocols.io.36wgq7b6kvk5/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.36wgq7b6kvk5/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.36wgq7b6kvk5/v1www.protocols.io/view/manual-dna-extraction-protocol-for-campylobacter-d-b88irzuewww.protocols.io/view/extraction-of-dna-from-canine-feces-for-detection-cg2ttyenprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.14egn286mg5d/v1

Extraction of DNA from canine feces for detection of Campylobacter jejuni using MagMaxTM CORE Nucleic Acid Purification Kit on KingFisherTM DuoPrime Instrument]]>Extraction of DNA from canine feces for detection of Campylobacter jejuni using MagMaxTM CORE Nucleic Acid Purification Kit on KingFisherTM DuoPrime InstrumentSara D. Lawhondx.doi.org/10.17504/protocols.io.14egn286mg5d/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.14egn286mg5d/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.14egn286mg5d/v1www.protocols.io/view/extraction-of-dna-from-canine-feces-for-detection-cg2ttyenwww.protocols.io/view/extraction-of-dna-from-canine-feces-for-detection-cg6rtzd6protocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.8epv5j1b5l1b/v1

Extraction of DNA from canine feces for detection of Campylobacter jejuni using the MagMaxTM CORE Nucleic Acid Purification Kit on KingFisherTM Flex Instrument]]>Extraction of DNA from canine feces for detection of Campylobacter jejuni using the MagMaxTM CORE Nucleic Acid Purification Kit on KingFisherTM Flex InstrumentSara D. Lawhondx.doi.org/10.17504/protocols.io.8epv5j1b5l1b/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.8epv5j1b5l1b/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.8epv5j1b5l1b/v1www.protocols.io/view/extraction-of-dna-from-canine-feces-for-detection-cg6rtzd6www.protocols.io/view/adna-extraction-cg8ttzwnprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.4r3l278p3g1y/v1

aDNA Extraction]]>aDNA ExtractionErnesto Garfiasdx.doi.org/10.17504/protocols.io.4r3l278p3g1y/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.4r3l278p3g1y/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.4r3l278p3g1y/v1www.protocols.io/view/adna-extraction-cg8ttzwnwww.protocols.io/view/limb-clasping-protocol-dain2cdeprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.6qpvr31q3vmk/v1

Limb clasping protocol]]>Limb clasping protocolHanne HD Dhondtdx.doi.org/10.17504/protocols.io.6qpvr31q3vmk/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.6qpvr31q3vmk/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.6qpvr31q3vmk/v1www.protocols.io/view/limb-clasping-protocol-dain2cdewww.protocols.io/view/tweap-protocol-for-target-enrichment-via-in-soluti-dbd82i9wprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.5qpvoknbxl4o/v1

Tweap protocol for target enrichment via in-solution hybridisation using Twist Bioscience Panels]]>Tweap protocol for target enrichment via in-solution hybridisation using Twist Bioscience PanelsTina Saupedx.doi.org/10.17504/protocols.io.5qpvoknbxl4o/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.5qpvoknbxl4o/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.5qpvoknbxl4o/v1www.protocols.io/view/tweap-protocol-for-target-enrichment-via-in-soluti-dbd82i9wwww.protocols.io/view/procedure-to-analyze-cannabinoids-in-bovine-urine-dgh93t96protocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.3byl495q8go5/v1

Procedure to analyze cannabinoids in bovine urine using solid phase extraction & UPLC MS/MS]]>Procedure to analyze cannabinoids in bovine urine using solid phase extraction & UPLC MS/MSGeraldine Magnindx.doi.org/10.17504/protocols.io.3byl495q8go5/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.3byl495q8go5/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.3byl495q8go5/v1www.protocols.io/view/procedure-to-analyze-cannabinoids-in-bovine-urine-dgh93t96www.protocols.io/view/3d-printed-social-place-preference-assays-for-aqua-dq645zgwprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.bp2l6d4y5vqe/v1

3D-printed social place preference assays for aquatic larvae]]>3D-printed social place preference assays for aquatic larvaeLauren A O'Connelldx.doi.org/10.17504/protocols.io.bp2l6d4y5vqe/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.bp2l6d4y5vqe/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.bp2l6d4y5vqe/v1www.protocols.io/view/3d-printed-social-place-preference-assays-for-aqua-dq645zgwwww.protocols.io/view/fluorescently-labeled-polyamine-uptake-via-flow-cy-dt6p6rdnprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.q26g7mpq1gwz/v1

Fluorescently labeled polyamine uptake (via flow cytometry)]]>Fluorescently labeled polyamine uptake (via flow cytometry)Sarah van Veendx.doi.org/10.17504/protocols.io.q26g7mpq1gwz/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.q26g7mpq1gwz/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.q26g7mpq1gwz/v1www.protocols.io/view/fluorescently-labeled-polyamine-uptake-via-flow-cy-dt6p6rdnwww.protocols.io/view/auto-de-phosphorylation-assay-dt6q6rdwprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.kxygxw3rwv8j/v1

Auto-(de)phosphorylation assay]]>Auto-(de)phosphorylation assaySarah van Veendx.doi.org/10.17504/protocols.io.kxygxw3rwv8j/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.kxygxw3rwv8j/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.kxygxw3rwv8j/v1www.protocols.io/view/auto-de-phosphorylation-assay-dt6q6rdwwww.protocols.io/view/measurement-of-atp13a4-atpase-activity-using-adp-g-dt7q6rmwprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.5qpvo9kxdv4o/v1

Measurement of ATP13A4 ATPase activity using ADP-Glo Max Assay]]>Measurement of ATP13A4 ATPase activity using ADP-Glo Max AssaySarah van Veendx.doi.org/10.17504/protocols.io.5qpvo9kxdv4o/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.5qpvo9kxdv4o/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.5qpvo9kxdv4o/v1www.protocols.io/view/measurement-of-atp13a4-atpase-activity-using-adp-g-dt7q6rmwwww.protocols.io/view/expression-and-purification-of-atp13a4-from-saccha-dt7v6rn6protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.ewov1d9jpvr2/v1

Expression and purification of ATP13A4 from Saccharomyces cerevisiae]]>Expression and purification of ATP13A4 from Saccharomyces cerevisiaeSarah van Veendx.doi.org/10.17504/protocols.io.ewov1d9jpvr2/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.ewov1d9jpvr2/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.ewov1d9jpvr2/v1www.protocols.io/view/expression-and-purification-of-atp13a4-from-saccha-dt7v6rn6www.protocols.io/view/purification-of-atp13a4-from-hek293t-cells-dt8d6rs6protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.dm6gp9z85vzp/v1

Purification of ATP13A4 from HEK293T cells]]>Purification of ATP13A4 from HEK293T cellsSarah van Veendx.doi.org/10.17504/protocols.io.dm6gp9z85vzp/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.dm6gp9z85vzp/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.dm6gp9z85vzp/v1www.protocols.io/view/purification-of-atp13a4-from-hek293t-cells-dt8d6rs6www.protocols.io/view/immunocytochemistry-subcellular-localization-of-at-dui86uhwprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.kxygxwy4kv8j/v1

Immunocytochemistry - subcellular localization of ATP13A4]]>Immunocytochemistry - subcellular localization of ATP13A4Sarah van Veendx.doi.org/10.17504/protocols.io.kxygxwy4kv8j/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.kxygxwy4kv8j/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.kxygxwy4kv8j/v1www.protocols.io/view/immunocytochemistry-subcellular-localization-of-at-dui86uhwwww.protocols.io/view/fluorescently-labeled-polyamine-uptake-via-confoca-dujg6ujwprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.5qpvo9kd9v4o/v1

Fluorescently labeled polyamine uptake (via confocal microscopy)]]>Fluorescently labeled polyamine uptake (via confocal microscopy)Sarah van Veendx.doi.org/10.17504/protocols.io.5qpvo9kd9v4o/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.5qpvo9kd9v4o/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.5qpvo9kd9v4o/v1www.protocols.io/view/fluorescently-labeled-polyamine-uptake-via-confoca-dujg6ujwwww.protocols.io/view/immunoblotting-dujt6unnprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.14egn96rql5d/v1

Immunoblotting]]>ImmunoblottingSarah van Veendx.doi.org/10.17504/protocols.io.14egn96rql5d/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.14egn96rql5d/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.14egn96rql5d/v1www.protocols.io/view/immunoblotting-dujt6unnwww.protocols.io/view/preparation-of-total-cell-lysates-duwj6xcnprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.eq2ly66wpgx9/v1

Preparation of total cell lysates]]>Preparation of total cell lysatesSarah van Veendx.doi.org/10.17504/protocols.io.eq2ly66wpgx9/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.eq2ly66wpgx9/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.eq2ly66wpgx9/v1www.protocols.io/view/preparation-of-total-cell-lysates-duwj6xcnwww.protocols.io/view/protein-quantification-by-sds-page-and-coomassie-s-duw86xhwprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.kqdg3qqqev25/v1

Protein quantification by SDS-PAGE and Coomassie staining]]>Protein quantification by SDS-PAGE and Coomassie stainingSarah van Veendx.doi.org/10.17504/protocols.io.kqdg3qqqev25/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.kqdg3qqqev25/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.kqdg3qqqev25/v1www.protocols.io/view/protein-quantification-by-sds-page-and-coomassie-s-duw86xhwwww.protocols.io/view/brain-slice-preparation-for-electrophysiology-reco-duyr6xv6protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.3byl4ww52vo5/v1

Brain slice preparation for electrophysiology recording]]>Brain slice preparation for electrophysiology recordingSarah van Veendx.doi.org/10.17504/protocols.io.3byl4ww52vo5/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.3byl4ww52vo5/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.3byl4ww52vo5/v1www.protocols.io/view/brain-slice-preparation-for-electrophysiology-reco-duyr6xv6www.protocols.io/view/postnatal-astrocyte-labeling-by-electroporation-pa-dxgb7jsnprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.n2bvj9z4xlk5/v1

Postnatal astrocyte labeling by electroporation (PALE)]]>Postnatal astrocyte labeling by electroporation (PALE)Sarah van Veendx.doi.org/10.17504/protocols.io.n2bvj9z4xlk5/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.n2bvj9z4xlk5/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.n2bvj9z4xlk5/v1www.protocols.io/view/postnatal-astrocyte-labeling-by-electroporation-pa-dxgb7jsnwww.protocols.io/view/synapse-staining-mouse-brain-sections-dxir7kd6protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.3byl4wxmzvo5/v1

Synapse staining - mouse brain sections]]>Synapse staining - mouse brain sectionsSarah van Veendx.doi.org/10.17504/protocols.io.3byl4wxmzvo5/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.3byl4wxmzvo5/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.3byl4wxmzvo5/v1www.protocols.io/view/synapse-staining-mouse-brain-sections-dxir7kd6www.protocols.io/view/rt-qpcr-cell-lines-dxmp7k5nprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.n92ldrq98g5b/v1

RT-qPCR (cell lines)]]>RT-qPCR (cell lines)Sarah van Veendx.doi.org/10.17504/protocols.io.n92ldrq98g5b/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.n92ldrq98g5b/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.n92ldrq98g5b/v1www.protocols.io/view/rt-qpcr-cell-lines-dxmp7k5nwww.protocols.io/view/analysis-of-vitamin-a-and-vitamin-e-in-serum-using-dzei73ceprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.261gerk5ol47/v1

Analysis of vitamin A and vitamin E in serum Using HPLC with UV and Fluorescence Detection ]]>Analysis of vitamin A and vitamin E in serum Using HPLC with UV and Fluorescence Detection Geraldine Magnindx.doi.org/10.17504/protocols.io.261gerk5ol47/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.261gerk5ol47/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.261gerk5ol47/v1www.protocols.io/view/analysis-of-vitamin-a-and-vitamin-e-in-serum-using-dzei73cewww.protocols.io/view/in-vivo-turboid-d5xt87nnprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.14egn4b9qv5d/v1

in vivo TurboID]]>in vivo TurboIDSarah van Veendx.doi.org/10.17504/protocols.io.14egn4b9qv5d/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.14egn4b9qv5d/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.14egn4b9qv5d/v1www.protocols.io/view/in-vivo-turboid-d5xt87nnwww.protocols.io/view/top10-chemically-competent-cells-g4s3bywgpprotocols.io, Published online: 2026-04-17; doi:

TOP10 chemically competent cells]]>TOP10 chemically competent cellsJiayi Houprotocols.io, Published online: 2026-04-17; | 2026-04-17protocols.iowww.protocols.io/view/top10-chemically-competent-cells-g4s3bywgpwww.protocols.io/view/halobacteriovorax-sp-gfr8-growth-and-aquarium-inoc-g6gfbzbtpprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.n2bvjexk5gk5/v1

Halobacteriovorax sp. GFR8 growth and aquarium inoculation protocol]]>Halobacteriovorax sp. GFR8 growth and aquarium inoculation protocolLauren Spearedx.doi.org/10.17504/protocols.io.n2bvjexk5gk5/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.n2bvjexk5gk5/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.n2bvjexk5gk5/v1www.protocols.io/view/halobacteriovorax-sp-gfr8-growth-and-aquarium-inoc-g6gfbzbtpwww.protocols.io/view/cortical-degradation-analysis-with-abba-g8u5bzwy7protocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.3byl46wjjgo5/v1

Cortical Degradation Analysis with ABBA]]>Cortical Degradation Analysis with ABBAJoseph Martindx.doi.org/10.17504/protocols.io.3byl46wjjgo5/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.3byl46wjjgo5/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.3byl46wjjgo5/v1www.protocols.io/view/cortical-degradation-analysis-with-abba-g8u5bzwy7www.protocols.io/view/nanopore-long-read-metabarcoding-for-the-comprehen-hc35b2yq7protocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.dm6gpmq1jgzp/v1

Nanopore long-read metabarcoding for the comprehensive characterisation of parasitic nematodes in humans and animals: a development and diagnostic validation study]]>Nanopore long-read metabarcoding for the comprehensive characterisation of parasitic nematodes in humans and animals: a development and diagnostic validation studyLucas Hugginsdx.doi.org/10.17504/protocols.io.dm6gpmq1jgzp/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.dm6gpmq1jgzp/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.dm6gpmq1jgzp/v1www.protocols.io/view/nanopore-long-read-metabarcoding-for-the-comprehen-hc35b2yq7www.protocols.io/view/a-simple-and-rapid-agarose-gel-electrophoresis-met-hgadb3sa7protocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.81wgbw3nqgpk/v1

A Simple and Rapid Agarose Gel Electrophoresis Method to Assess CpG Methylation of DNA]]>A Simple and Rapid Agarose Gel Electrophoresis Method to Assess CpG Methylation of DNAMarco Paliza-Carredx.doi.org/10.17504/protocols.io.81wgbw3nqgpk/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.81wgbw3nqgpk/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.81wgbw3nqgpk/v1www.protocols.io/view/a-simple-and-rapid-agarose-gel-electrophoresis-met-hgadb3sa7www.protocols.io/view/gift-seq-nuclei-preparation-hhjmb34k7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.8epv5kwkdv1b/v1

GIFT-seq: Nuclei Preparation]]>GIFT-seq: Nuclei PreparationKarolis Kumpaitisdx.doi.org/10.17504/protocols.io.8epv5kwkdv1b/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.8epv5kwkdv1b/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.8epv5kwkdv1b/v1www.protocols.io/view/gift-seq-nuclei-preparation-hhjmb34k7www.protocols.io/view/gift-seq-gapfill-and-library-preparation-protocol-hhz3b378pprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.4r3l2zm3jl1y/v1

GIFT-seq: Gapfill and Library Preparation Protocol]]>GIFT-seq: Gapfill and Library Preparation ProtocolKarolis Kumpaitisdx.doi.org/10.17504/protocols.io.4r3l2zm3jl1y/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.4r3l2zm3jl1y/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.4r3l2zm3jl1y/v1www.protocols.io/view/gift-seq-gapfill-and-library-preparation-protocol-hhz3b378pwww.protocols.io/view/measurements-of-the-condylar-surface-area-of-tmj-i-hingb4dbxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.kqdg3n4wev25/v1

Measurements of the condylar surface area of TMJ in 3D]]>Measurements of the condylar surface area of TMJ in 3DAnna Walczakdx.doi.org/10.17504/protocols.io.kqdg3n4wev25/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.kqdg3n4wev25/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.kqdg3n4wev25/v1www.protocols.io/view/measurements-of-the-condylar-surface-area-of-tmj-i-hingb4dbxwww.protocols.io/view/perineuronal-net-mapping-and-quantification-with-q-hmaeb42bfprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.261ge1ddyv47/v1

Perineuronal Net Mapping and Quantification with Quint]]>Perineuronal Net Mapping and Quantification with QuintJoseph Martindx.doi.org/10.17504/protocols.io.261ge1ddyv47/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.261ge1ddyv47/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.261ge1ddyv47/v1www.protocols.io/view/perineuronal-net-mapping-and-quantification-with-q-hmaeb42bfwww.protocols.io/view/ideel-unc-implementation-of-targeted-csp-sequencin-hp8rb5rv7protocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.8epv55xz5v1b/v2

IDEEL- UNC Implementation of Targeted csp Sequencing- MIP to Nanopore]]>IDEEL- UNC Implementation of Targeted csp Sequencing- MIP to NanoporeInfectious Disease Epidemiology and Ecology Labdx.doi.org/10.17504/protocols.io.8epv55xz5v1b/v2protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.8epv55xz5v1b/v22026-04-14protocols.iodx.doi.org/10.17504/protocols.io.8epv55xz5v1b/v2www.protocols.io/view/ideel-unc-implementation-of-targeted-csp-sequencin-hp8rb5rv7www.protocols.io/view/ambrosia-beetle-surface-sterilization-hqxab5xifprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.x54v9bk44l3e/v1

AMBROSIA BEETLE SURFACE STERILIZATION]]>AMBROSIA BEETLE SURFACE STERILIZATIONM. Elizabeth Mooredx.doi.org/10.17504/protocols.io.x54v9bk44l3e/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.x54v9bk44l3e/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.x54v9bk44l3e/v1www.protocols.io/view/ambrosia-beetle-surface-sterilization-hqxab5xifwww.protocols.io/view/enriched-seawater-for-cyanobacterial-culture-hq7qb5zmxprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.5qpvo183xg4o/v1

Enriched Seawater for Cyanobacterial Culture]]>Enriched Seawater for Cyanobacterial CultureRômulo Pereira de Jesusdx.doi.org/10.17504/protocols.io.5qpvo183xg4o/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.5qpvo183xg4o/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.5qpvo183xg4o/v1www.protocols.io/view/enriched-seawater-for-cyanobacterial-culture-hq7qb5zmxwww.protocols.io/view/bidmc-tmc-stu-short-read-sequencing-pe-2x100-on-si-hsssb6eefprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.rm7vze988vx1/v1

BIDMC TMC / STU - Short read sequencing (PE 2x100) on Singular Genomics G4 NGS sequencer (Version 2026)]]>BIDMC TMC / STU - Short read sequencing (PE 2x100) on Singular Genomics G4 NGS sequencer (Version 2026)Shuoshuo Wangdx.doi.org/10.17504/protocols.io.rm7vze988vx1/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.rm7vze988vx1/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.rm7vze988vx1/v1www.protocols.io/view/bidmc-tmc-stu-short-read-sequencing-pe-2x100-on-si-hsssb6eefwww.protocols.io/view/method-for-enhancing-reprogramming-towards-induced-huqsb6vwfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.6qpvrynrzgmk/v1

Method for enhancing reprogramming towards induced human expanded pluripotency with engineered SOX17 transcription factors]]>Method for enhancing reprogramming towards induced human expanded pluripotency with engineered SOX17 transcription factorsHaoqing HUdx.doi.org/10.17504/protocols.io.6qpvrynrzgmk/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.6qpvrynrzgmk/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.6qpvrynrzgmk/v1www.protocols.io/view/method-for-enhancing-reprogramming-towards-induced-huqsb6vwfwww.protocols.io/view/txtperpus-curation-workflow-jws2cpegfprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.e6nvww4x7vmk/v1

TXTperpus Curation Workflow]]>TXTperpus Curation WorkflowFaizhal Arif Faizhal Santosadx.doi.org/10.17504/protocols.io.e6nvww4x7vmk/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.e6nvww4x7vmk/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.e6nvww4x7vmk/v1www.protocols.io/view/txtperpus-curation-workflow-jws2cpegfwww.protocols.io/view/library-preparation-of-sars-cov-2-rna-using-molecu-jxascpiefprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.36wgqx18klk5/v1

Library preparation of SARS-CoV-2 RNA using Molecular Inversion Probes (MIPs) target capture for Whole Genome Sequencing (WGS) on Illumina platform]]>Library preparation of SARS-CoV-2 RNA using Molecular Inversion Probes (MIPs) target capture for Whole Genome Sequencing (WGS) on Illumina platformHortensia-Clara Radulescudx.doi.org/10.17504/protocols.io.36wgqx18klk5/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.36wgqx18klk5/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.36wgqx18klk5/v1www.protocols.io/view/library-preparation-of-sars-cov-2-rna-using-molecu-jxascpiefwww.protocols.io/view/ihc-staining-dab-of-iba1-and-gfap-jxftcpjnpprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.q26g7oj83vwz/v1

IHC staining (DAB) of Iba1 and Gfap]]>IHC staining (DAB) of Iba1 and GfapSarah van Veendx.doi.org/10.17504/protocols.io.q26g7oj83vwz/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.q26g7oj83vwz/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.q26g7oj83vwz/v1www.protocols.io/view/ihc-staining-dab-of-iba1-and-gfap-jxftcpjnpwww.protocols.io/view/implementation-of-hpv-self-sampling-in-cervical-sc-jxj2cpkqfprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v1

Implementation of HPV Self-Sampling in Cervical Screening: A Scoping Review of Barriers, Facilitators and Key Challenges (protocol)]]>Implementation of HPV Self-Sampling in Cervical Screening: A Scoping Review of Barriers, Facilitators and Key Challenges (protocol)Daisy Urquhart-Dixondx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v1www.protocols.io/view/implementation-of-hpv-self-sampling-in-cervical-sc-jxj2cpkqfwww.protocols.io/view/protocol-for-cleaning-and-preparing-insect-specime-jxj7cpkrpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.4r3l2dr6qg1y/v1

Protocol for Cleaning and Preparing Insect Specimens Trapped into Adhesive (e.g., TAD, Tanglefoot, Pestik) Prior to Diagnostic Identification and DNA Extraction]]>Protocol for Cleaning and Preparing Insect Specimens Trapped into Adhesive (e.g., TAD, Tanglefoot, Pestik) Prior to Diagnostic Identification and DNA ExtractionAxel David Gonzalez Murillodx.doi.org/10.17504/protocols.io.4r3l2dr6qg1y/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.4r3l2dr6qg1y/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.4r3l2dr6qg1y/v1www.protocols.io/view/protocol-for-cleaning-and-preparing-insect-specime-jxj7cpkrpwww.protocols.io/view/library-preparation-using-the-arima-library-prep-m-jxkkcpkuxprotocols.io, Published online: 2026-04-15; doi:

Library Preparation using the Arima Library Prep Module]]>Library Preparation using the Arima Library Prep ModuleAndrew Kaoprotocols.io, Published online: 2026-04-15; | 2026-04-15protocols.iowww.protocols.io/view/library-preparation-using-the-arima-library-prep-m-jxkkcpkuxwww.protocols.io/view/6-polyacrylamide-gel-tbe-system-jxnacpmafprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.e6nvww7o9vmk/v1

6% Polyacrylamide Gel (TBE system)]]> 6% Polyacrylamide Gel (TBE system)Md Jubayer Alamdx.doi.org/10.17504/protocols.io.e6nvww7o9vmk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.e6nvww7o9vmk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.e6nvww7o9vmk/v1www.protocols.io/view/6-polyacrylamide-gel-tbe-system-jxnacpmafwww.protocols.io/view/recombinant-human-egfr-his-and-myc-tagged-low-endo-jxnzcpmf7protocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.81wgbj8q1vpk/v1

Recombinant Human EGFR His and Myc Tagged, low endotoxin (CSB-MP007479HU) ELISA Protocol]]>Recombinant Human EGFR His and Myc Tagged, low endotoxin (CSB-MP007479HU) ELISA ProtocolRosie Liudx.doi.org/10.17504/protocols.io.81wgbj8q1vpk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.81wgbj8q1vpk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.81wgbj8q1vpk/v1www.protocols.io/view/recombinant-human-egfr-his-and-myc-tagged-low-endo-jxnzcpmf7www.protocols.io/view/lamp-protocol-for-entomopathogenic-serratia-spp-in-jxqccpmsxprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.bp2l69j7zlqe/v2

LAMP Protocol for Entomopathogenic Serratia spp. in Insect Frass - 2026 Update with Tissue Homogeniser]]>LAMP Protocol for Entomopathogenic Serratia spp. in Insect Frass - 2026 Update with Tissue HomogeniserNicholas P Doidgedx.doi.org/10.17504/protocols.io.bp2l69j7zlqe/v2protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.bp2l69j7zlqe/v22026-04-13protocols.iodx.doi.org/10.17504/protocols.io.bp2l69j7zlqe/v2www.protocols.io/view/lamp-protocol-for-entomopathogenic-serratia-spp-in-jxqccpmsxwww.protocols.io/view/collection-of-mouse-cerebrospinal-fluid-from-the-c-jxqhcpmt7protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.81wgbj8k1vpk/v1

Collection of mouse cerebrospinal fluid from the cisterna magna]]>Collection of mouse cerebrospinal fluid from the cisterna magnaSarah van Veendx.doi.org/10.17504/protocols.io.81wgbj8k1vpk/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.81wgbj8k1vpk/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.81wgbj8k1vpk/v1www.protocols.io/view/collection-of-mouse-cerebrospinal-fluid-from-the-c-jxqhcpmt7www.protocols.io/view/brain-dissection-protocol-jxqkcpmuxprotocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.yxmvm8jmng3p/v1

Brain dissection protocol]]>Brain dissection protocolSarah van Veendx.doi.org/10.17504/protocols.io.yxmvm8jmng3p/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.yxmvm8jmng3p/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.yxmvm8jmng3p/v1www.protocols.io/view/brain-dissection-protocol-jxqkcpmuxwww.protocols.io/view/the-irrelevant-babble-effect-in-individuals-with-a-jxqucpmwxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.kqdg3mdw7l25/v1

The irrelevant babble effect in individuals with autism spectrum disorder]]>The irrelevant babble effect in individuals with autism spectrum disorderLejla Alikadicdx.doi.org/10.17504/protocols.io.kqdg3mdw7l25/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.kqdg3mdw7l25/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.kqdg3mdw7l25/v1www.protocols.io/view/the-irrelevant-babble-effect-in-individuals-with-a-jxqucpmwxwww.protocols.io/view/intrusion-detection-latency-the-neglected-metric-jxqycpmxxprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.bp2l6jwyzvqe/v1

Intrusion Detection Latency : The Neglected Metric]]>Intrusion Detection Latency : The Neglected MetricSandhyarani Dashdx.doi.org/10.17504/protocols.io.bp2l6jwyzvqe/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.bp2l6jwyzvqe/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.bp2l6jwyzvqe/v1www.protocols.io/view/intrusion-detection-latency-the-neglected-metric-jxqycpmxxwww.protocols.io/view/subsampling-bones-and-teeth-for-ancient-dna-extrac-jxrxcpm7pprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.q26g7n5o3lwz/v2

Subsampling bones and teeth for ancient DNA extraction]]>Subsampling bones and teeth for ancient DNA extractionDeon de Jagerdx.doi.org/10.17504/protocols.io.q26g7n5o3lwz/v2protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.q26g7n5o3lwz/v22026-04-13protocols.iodx.doi.org/10.17504/protocols.io.q26g7n5o3lwz/v2www.protocols.io/view/subsampling-bones-and-teeth-for-ancient-dna-extrac-jxrxcpm7pwww.protocols.io/view/detection-of-low-frequency-mutations-quasispecies-jxr7cpm9pprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.n92ld4e18l5b/v1

Detection of Low-Frequency Mutations (Quasispecies Diversity) in RNA Viral Genome Sequencing Data]]>Detection of Low-Frequency Mutations (Quasispecies Diversity) in RNA Viral Genome Sequencing DataVivek T Natarajandx.doi.org/10.17504/protocols.io.n92ld4e18l5b/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.n92ld4e18l5b/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.n92ld4e18l5b/v1www.protocols.io/view/detection-of-low-frequency-mutations-quasispecies-jxr7cpm9pwww.protocols.io/view/protocols-for-34-lrrk2-mutations-block-ncoa4-traff-jxsicpncfprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.x54v9q4b4l3e/v1

Protocols for "LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death"]]>Protocols for "LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death"Lilia Rodriguezdx.doi.org/10.17504/protocols.io.x54v9q4b4l3e/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.x54v9q4b4l3e/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.x54v9q4b4l3e/v1www.protocols.io/view/protocols-for-34-lrrk2-mutations-block-ncoa4-traff-jxsicpncfwww.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jxsrcpnd7protocols.io, Published online: 2026-04-14; doi:

Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seq]]>Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seqGreggory A A Perryprotocols.io, Published online: 2026-04-14; | 2026-04-14protocols.iowww.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jxsrcpnd7www.protocols.io/view/user-ii-treatment-of-ancient-dna-extracts-jxsucpnexprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.eq2ly6qrwgx9/v2

USER II treatment of ancient DNA extracts]]>USER II treatment of ancient DNA extractsDeon de Jagerdx.doi.org/10.17504/protocols.io.eq2ly6qrwgx9/v2protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.eq2ly6qrwgx9/v22026-04-14protocols.iodx.doi.org/10.17504/protocols.io.eq2ly6qrwgx9/v2www.protocols.io/view/user-ii-treatment-of-ancient-dna-extracts-jxsucpnexwww.protocols.io/view/cell-culture-transfection-and-immunoblotting-jxsvcpne7protocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.81wgbj88yvpk/v1

Cell Culture Transfection and Immunoblotting]]>Cell Culture Transfection and ImmunoblottingJacquelyn Haytayandx.doi.org/10.17504/protocols.io.81wgbj88yvpk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.81wgbj88yvpk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.81wgbj88yvpk/v1www.protocols.io/view/cell-culture-transfection-and-immunoblotting-jxsvcpne7www.protocols.io/view/immunofluorescent-image-acquisition-and-quantifica-jxs4cpngxprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.5qpvoe447l4o/v1

Immunofluorescent Image Acquisition and Quantification]]>Immunofluorescent Image Acquisition and QuantificationJacquelyn Haytayandx.doi.org/10.17504/protocols.io.5qpvoe447l4o/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.5qpvoe447l4o/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.5qpvoe447l4o/v1www.protocols.io/view/immunofluorescent-image-acquisition-and-quantifica-jxs4cpngxwww.protocols.io/view/immunoperoxidase-histology-and-densitometric-quant-jxs6cpnhfprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.36wgqxkkylk5/v1

Immunoperoxidase Histology and Densitometric Quantification for Tyrosine Hydroxylase]]>Immunoperoxidase Histology and Densitometric Quantification for Tyrosine HydroxylaseJacquelyn Haytayandx.doi.org/10.17504/protocols.io.36wgqxkkylk5/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.36wgqxkkylk5/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.36wgqxkkylk5/v1www.protocols.io/view/immunoperoxidase-histology-and-densitometric-quant-jxs6cpnhfwww.protocols.io/view/bulk-rna-seq-protocol-for-astrocytes-jxtmcpnk7protocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.81wgbj8eyvpk/v1

Bulk RNA-seq Protocol for Astrocytes]]>Bulk RNA-seq Protocol for AstrocytesElena Cocciadx.doi.org/10.17504/protocols.io.81wgbj8eyvpk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.81wgbj8eyvpk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.81wgbj8eyvpk/v1www.protocols.io/view/bulk-rna-seq-protocol-for-astrocytes-jxtmcpnk7www.protocols.io/view/atac-seq-protocol-for-astrocytes-jxtncpnmfprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.6qpvrbzn3lmk/v1

ATAC-seq Protocol for Astrocytes ]]>ATAC-seq Protocol for Astrocytes Elena Cocciadx.doi.org/10.17504/protocols.io.6qpvrbzn3lmk/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.6qpvrbzn3lmk/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.6qpvrbzn3lmk/v1www.protocols.io/view/atac-seq-protocol-for-astrocytes-jxtncpnmfwww.protocols.io/view/bisulfite-seq-for-astrocytes-jxtpcpnmpprotocols.io, Published online: 2026-04-13; doi:dx.doi.org/10.17504/protocols.io.n92ld4ex8l5b/v1

Bisulfite-seq for Astrocytes]]>Bisulfite-seq for AstrocytesElena Cocciadx.doi.org/10.17504/protocols.io.n92ld4ex8l5b/v1protocols.io, Published online: 2026-04-13; | dx.doi.org/10.17504/protocols.io.n92ld4ex8l5b/v12026-04-13protocols.iodx.doi.org/10.17504/protocols.io.n92ld4ex8l5b/v1www.protocols.io/view/bisulfite-seq-for-astrocytes-jxtpcpnmpwww.protocols.io/view/engineering-the-developmental-continuum-from-embry-jxttcpnnpprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.1007/7651_2026_700

Engineering the Developmental Continuum: From Embryo-Like Structures to Functional Tissue Interfaces]]>Engineering the Developmental Continuum: From Embryo-Like Structures to Functional Tissue InterfacesProtocols Importerdx.doi.org/10.1007/7651_2026_700protocols.io, Published online: 2026-04-14; | dx.doi.org/10.1007/7651_2026_7002026-04-14protocols.iodx.doi.org/10.1007/7651_2026_700www.protocols.io/view/engineering-the-developmental-continuum-from-embry-jxttcpnnpwww.protocols.io/view/crystallization-of-zika-virus-ns2b-ns3-protease-cl-jxuhcpnt7protocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.dm6gp76ndgzp/v1

Crystallization of Zika virus NS2B-NS3 protease (closed conformation in space group P4322 | PDB ID: 8PN6)]]>Crystallization of Zika virus NS2B-NS3 protease (closed conformation in space group P4322 | PDB ID: 8PN6)Peter Marplesdx.doi.org/10.17504/protocols.io.dm6gp76ndgzp/v1protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.dm6gp76ndgzp/v12026-04-14protocols.iodx.doi.org/10.17504/protocols.io.dm6gp76ndgzp/v1www.protocols.io/view/crystallization-of-zika-virus-ns2b-ns3-protease-cl-jxuhcpnt7www.protocols.io/view/assessing-protein-crystal-tolerance-of-chemistry-r-jxupcpnvpprotocols.io, Published online: 2026-04-14; doi:dx.doi.org/10.17504/protocols.io.14egn516yg5d/v2

Assessing protein crystal tolerance of chemistry reagents for HT-crystallographic screening]]>Assessing protein crystal tolerance of chemistry reagents for HT-crystallographic screeningMilo Cooperdx.doi.org/10.17504/protocols.io.14egn516yg5d/v2protocols.io, Published online: 2026-04-14; | dx.doi.org/10.17504/protocols.io.14egn516yg5d/v22026-04-14protocols.iodx.doi.org/10.17504/protocols.io.14egn516yg5d/v2www.protocols.io/view/assessing-protein-crystal-tolerance-of-chemistry-r-jxupcpnvpwww.protocols.io/view/cardiovascular-health-and-lifestyle-in-arag-n-adol-jxu3cpnypprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.14egn5odzg5d/v1

Cardiovascular Health and Lifestyle in Aragón Adolescents (CHIARA): protocol for a longitudinal cohort study integrating cardiovascular, behavioural and psychosocial assessment]]>Cardiovascular Health and Lifestyle in Aragón Adolescents (CHIARA): protocol for a longitudinal cohort study integrating cardiovascular, behavioural and psychosocial assessmentIris Iglesia Altabadx.doi.org/10.17504/protocols.io.14egn5odzg5d/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.14egn5odzg5d/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.14egn5odzg5d/v1www.protocols.io/view/cardiovascular-health-and-lifestyle-in-arag-n-adol-jxu3cpnypwww.protocols.io/view/implementation-of-hpv-self-sampling-in-cervical-sc-jxwrcppd7protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v2

Implementation of HPV Self-Sampling in Cervical Screening: A Scoping Review of Barriers, Facilitators and Key Challenges (protocol)]]>Implementation of HPV Self-Sampling in Cervical Screening: A Scoping Review of Barriers, Facilitators and Key Challenges (protocol)Daisy Urquhart-Dixondx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v2protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v22026-04-15protocols.iodx.doi.org/10.17504/protocols.io.e6nvww7e9vmk/v2www.protocols.io/view/implementation-of-hpv-self-sampling-in-cervical-sc-jxwrcppd7www.protocols.io/view/facial-vein-blood-collection-jxxdcppi7protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.8epv5yq7jl1b/v1

Facial vein blood collection]]>Facial vein blood collectionSarah van Veendx.doi.org/10.17504/protocols.io.8epv5yq7jl1b/v1protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.8epv5yq7jl1b/v12026-04-15protocols.iodx.doi.org/10.17504/protocols.io.8epv5yq7jl1b/v1www.protocols.io/view/facial-vein-blood-collection-jxxdcppi7www.protocols.io/view/mouse-heart-nuclei-isolation-10x-genomics-nuclei-i-jxxscppnfprotocols.io, Published online: 2026-04-16; doi:

Mouse Heart Nuclei Isolation – 10x Genomics Nuclei Isolation Kit- Miltenyi Anti-Nucleus Beads – 10X Genomics scRNAseq, Multiome]]>Mouse Heart Nuclei Isolation – 10x Genomics Nuclei Isolation Kit- Miltenyi Anti-Nucleus Beads – 10X Genomics scRNAseq, MultiomeGreggory A A Perryprotocols.io, Published online: 2026-04-16; | 2026-04-16protocols.iowww.protocols.io/view/mouse-heart-nuclei-isolation-10x-genomics-nuclei-i-jxxscppnfwww.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jxxwcpppfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v2

Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seq]]>Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seqGreggory A A Perrydx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v2protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v22026-04-16protocols.iodx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v2www.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jxxwcpppfwww.protocols.io/view/hidef-seq-jxx4cppqxprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.kxygxy9mwl8j/v6

HiDEF-seq]]>HiDEF-seqGilad Evronydx.doi.org/10.17504/protocols.io.kxygxy9mwl8j/v6protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.kxygxy9mwl8j/v62026-04-17protocols.iodx.doi.org/10.17504/protocols.io.kxygxy9mwl8j/v6www.protocols.io/view/hidef-seq-jxx4cppqxwww.protocols.io/view/smarterv4-1x-amplification-for-single-cell-or-sing-jxx5cppq7protocols.io, Published online: 2026-04-15; doi:dx.doi.org/10.17504/protocols.io.261geopdjl47/v5

SMARTerV4 (1x) Amplification for single-cell or single-nuclei RNASeq Protocol]]>SMARTerV4 (1x) Amplification for single-cell or single-nuclei RNASeq ProtocolAllen Institutedx.doi.org/10.17504/protocols.io.261geopdjl47/v5protocols.io, Published online: 2026-04-15; | dx.doi.org/10.17504/protocols.io.261geopdjl47/v52026-04-15protocols.iodx.doi.org/10.17504/protocols.io.261geopdjl47/v5www.protocols.io/view/smarterv4-1x-amplification-for-single-cell-or-sing-jxx5cppq7www.protocols.io/view/effect-of-electronic-nursing-documentation-systems-jxyhcppt7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.dm6gp7k81gzp/v1

Effect of Electronic Nursing Documentation Systems on Documentation Completeness and Patient Safety Outcomes: A Systematic Review and Meta-Analysis]]>Effect of Electronic Nursing Documentation Systems on Documentation Completeness and Patient Safety Outcomes: A Systematic Review and Meta-Analysisaljomaie.hassandx.doi.org/10.17504/protocols.io.dm6gp7k81gzp/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.dm6gp7k81gzp/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.dm6gp7k81gzp/v1www.protocols.io/view/effect-of-electronic-nursing-documentation-systems-jxyhcppt7www.protocols.io/view/strategies-for-the-conservation-of-plant-genetic-r-jxyicppufprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_2

Strategies for the Conservation of Plant Genetic Resources]]>Strategies for the Conservation of Plant Genetic ResourcesProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_2protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_22026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_2www.protocols.io/view/strategies-for-the-conservation-of-plant-genetic-r-jxyicppufwww.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxykcppuxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_20

Medium-Term Preservation by the In Vitro Minimum Growth Method of Swietenia humilis Zucc]]>Medium-Term Preservation by the In Vitro Minimum Growth Method of Swietenia humilis ZuccProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_20protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_202026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_20www.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxykcppuxwww.protocols.io/view/in-vitro-conservation-of-em-agave-potatorum-em-zuc-jxymcppu7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_18

In Vitro Conservation of Agave potatorum Zucc and Agave marmorata Roezl]]>In Vitro Conservation of Agave potatorum Zucc and Agave marmorata RoezlProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_18protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_182026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_18www.protocols.io/view/in-vitro-conservation-of-em-agave-potatorum-em-zuc-jxymcppu7www.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxyncppvfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_23

Long-Term Preservation by the Cryopreservation Method of Cordia alliodora (Ruiz & Pavón) Oken]]>Long-Term Preservation by the Cryopreservation Method of Cordia alliodora (Ruiz & Pavón) OkenProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_23protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_232026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_23www.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxyncppvfwww.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxypcppvpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_25

Long-Term Preservation by the Cryopreservation Method Cedrela odorata L.]]>Long-Term Preservation by the Cryopreservation Method Cedrela odorata L.Protocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_25protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_252026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_25www.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxypcppvpwww.protocols.io/view/germination-protocol-for-cedar-walnut-seeds-em-jug-jxyqcppvxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_4

Germination Protocol for Cedar Walnut Seeds (Juglans pyriformis Liebm.) Using Thermal Shock]]>Germination Protocol for Cedar Walnut Seeds (Juglans pyriformis Liebm.) Using Thermal ShockProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_4protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_42026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_4www.protocols.io/view/germination-protocol-for-cedar-walnut-seeds-em-jug-jxyqcppvxwww.protocols.io/view/long-term-storage-of-vanilla-em-vanilla-planifolia-jxyrcppv7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_24

Long-Term Storage of Vanilla (Vanilla planifolia Jacks.) by Cryopreservation Method]]>Long-Term Storage of Vanilla (Vanilla planifolia Jacks.) by Cryopreservation MethodProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_24protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_242026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_24www.protocols.io/view/long-term-storage-of-vanilla-em-vanilla-planifolia-jxyrcppv7www.protocols.io/view/advances-in-cryogenic-techniques-for-the-in-vitro-jxyscppwfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_30

Advances in Cryogenic Techniques for the In Vitro Preservation of Somatic Embryos and Shoot Tips of Plants]]>Advances in Cryogenic Techniques for the In Vitro Preservation of Somatic Embryos and Shoot Tips of PlantsProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_30protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_302026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_30www.protocols.io/view/advances-in-cryogenic-techniques-for-the-in-vitro-jxyscppwfwww.protocols.io/view/in-situ-conservation-of-agricultural-biodiversity-jxytcppwpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_3

In Situ Conservation of Agricultural Biodiversity and Generation of Projects in Ejidos and Communities in Mexico]]>In Situ Conservation of Agricultural Biodiversity and Generation of Projects in Ejidos and Communities in MexicoProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_3protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_32026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_3www.protocols.io/view/in-situ-conservation-of-agricultural-biodiversity-jxytcppwpwww.protocols.io/view/biotechnological-tools-for-em-agave-i-conservation-jxyucppwxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_29

Biotechnological Tools for Agave Conservation]]>Biotechnological Tools for Agave ConservationProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_29protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_292026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_29www.protocols.io/view/biotechnological-tools-for-em-agave-i-conservation-jxyucppwxwww.protocols.io/view/in-vitro-storage-techniques-for-orchids-conservati-jxyvcppw7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_21

In Vitro Storage Techniques for Orchids Conservation: A Review]]>In Vitro Storage Techniques for Orchids Conservation: A ReviewProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_21protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_212026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_21www.protocols.io/view/in-vitro-storage-techniques-for-orchids-conservati-jxyvcppw7www.protocols.io/view/perspectives-on-plant-germplasm-conservation-and-b-jxywcppxfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_34

Perspectives on Plant Germplasm Conservation and Biobanking]]>Perspectives on Plant Germplasm Conservation and BiobankingProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_34protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_342026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_34www.protocols.io/view/perspectives-on-plant-germplasm-conservation-and-b-jxywcppxfwww.protocols.io/view/evaluation-and-short-term-conservation-of-em-thrin-jxyxcppxpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_5

Evaluation and Short-Term Conservation of Thrinax radiata (Arecaceae) seeds]]>Evaluation and Short-Term Conservation of Thrinax radiata (Arecaceae) seedsProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_5protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_52026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_5www.protocols.io/view/evaluation-and-short-term-conservation-of-em-thrin-jxyxcppxpwww.protocols.io/view/osmotic-conditioning-of-amaranth-seeds-to-increase-jxyycppxxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_8

Osmotic Conditioning of Amaranth Seeds to Increase Germination]]>Osmotic Conditioning of Amaranth Seeds to Increase GerminationProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_8protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_82026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_8www.protocols.io/view/osmotic-conditioning-of-amaranth-seeds-to-increase-jxyycppxxwww.protocols.io/view/factors-influencing-in-vitro-conservation-of-em-va-jxyzcppx7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_22

Factors Influencing In Vitro Conservation of Vanilla Species in Minimal Growth]]>Factors Influencing In Vitro Conservation of Vanilla Species in Minimal GrowthProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_22protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_222026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_22www.protocols.io/view/factors-influencing-in-vitro-conservation-of-em-va-jxyzcppx7www.protocols.io/view/ex-situ-conservation-of-epiphytic-orchids-a-biblio-jxy2cppyfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_13

Ex Situ Conservation of Epiphytic Orchids: A Bibliometric Review]]>Ex Situ Conservation of Epiphytic Orchids: A Bibliometric ReviewProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_13protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_132026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_13www.protocols.io/view/ex-situ-conservation-of-epiphytic-orchids-a-biblio-jxy2cppyfwww.protocols.io/view/germination-of-em-calibanus-hookeri-i-lem-trel-see-jxy3cppypprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_7

Germination of Calibanus hookeri (Lem.) Trel. Seeds Through Imbibition in Distilled Water Plus Physical Scarification]]>Germination of Calibanus hookeri (Lem.) Trel. Seeds Through Imbibition in Distilled Water Plus Physical ScarificationProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_7protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_72026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_7www.protocols.io/view/germination-of-em-calibanus-hookeri-i-lem-trel-see-jxy3cppypwww.protocols.io/view/ex-situ-conservation-of-em-opuntia-i-spp-germplasm-jxy4cppyxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_12

Ex Situ Conservation of Opuntia spp. Germplasm in a Complementary Agriculture Model]]>Ex Situ Conservation of Opuntia spp. Germplasm in a Complementary Agriculture ModelProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_12protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_122026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_12www.protocols.io/view/ex-situ-conservation-of-em-opuntia-i-spp-germplasm-jxy4cppyxwww.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxy5cppy7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_17

Medium-Term Preservation by the In Vitro Minimum Growth Method of Gmelina arborea Roxb]]>Medium-Term Preservation by the In Vitro Minimum Growth Method of Gmelina arborea RoxbProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_17protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_172026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_17www.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxy5cppy7www.protocols.io/view/asymbiotic-germination-micropropagation-and-slow-g-jxy6cppzfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_19

Asymbiotic Germination, Micropropagation, and Slow Growth In Vitro Culture for Conservation of Epidendrum stamfordianum Bateman]]>Asymbiotic Germination, Micropropagation, and Slow Growth In Vitro Culture for Conservation of Epidendrum stamfordianum BatemanProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_19protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_192026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_19www.protocols.io/view/asymbiotic-germination-micropropagation-and-slow-g-jxy6cppzfwww.protocols.io/view/overview-of-plant-genetic-resources-conservation-jxy7cppzpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_1

Overview of Plant Genetic Resources Conservation]]>Overview of Plant Genetic Resources ConservationProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_12026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_1www.protocols.io/view/overview-of-plant-genetic-resources-conservation-jxy7cppzpwww.protocols.io/view/short-term-conservation-and-exploitation-of-sweet-jxy8cppzxprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_6

Short-Term Conservation and Exploitation of Sweet Acacia (Vachellia farnesiana L. Wight & Arn.) Through an Efficient Scarification-Based Germination Protocol]]>Short-Term Conservation and Exploitation of Sweet Acacia (Vachellia farnesiana L. Wight & Arn.) Through an Efficient Scarification-Based Germination ProtocolProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_6protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_62026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_6www.protocols.io/view/short-term-conservation-and-exploitation-of-sweet-jxy8cppzxwww.protocols.io/view/uses-and-conservation-of-chaya-in-the-southeast-of-jxy9cppz7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_10

Uses and Conservation of Chaya in the Southeast of Mexico]]>Uses and Conservation of Chaya in the Southeast of MexicoProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_10protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_102026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_10www.protocols.io/view/uses-and-conservation-of-chaya-in-the-southeast-of-jxy9cppz7www.protocols.io/view/in-vitro-preservation-of-vanilla-em-vanila-planifo-jxzacpp2fprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_15

In Vitro Preservation of Vanilla (Vanila planifolia Jacks. Ex Andrew) by Slow Growth]]>In Vitro Preservation of Vanilla (Vanila planifolia Jacks. Ex Andrew) by Slow GrowthProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_15protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_152026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_15www.protocols.io/view/in-vitro-preservation-of-vanilla-em-vanila-planifo-jxzacpp2fwww.protocols.io/view/plant-tissue-culture-a-short-term-conservation-too-jxzbcpp2pprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_11

Plant Tissue Culture: A Short-Term Conservation Tool]]>Plant Tissue Culture: A Short-Term Conservation ToolProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_11protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_112026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_11www.protocols.io/view/plant-tissue-culture-a-short-term-conservation-too-jxzbcpp2pwww.protocols.io/view/omics-sciences-driving-the-conservation-and-charac-jxzccpp2xprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_32

Omics Sciences: Driving the Conservation and Characterization of Plant Genetic Resources]]>Omics Sciences: Driving the Conservation and Characterization of Plant Genetic ResourcesProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_32protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_322026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_32www.protocols.io/view/omics-sciences-driving-the-conservation-and-charac-jxzccpp2xwww.protocols.io/view/perspectives-of-gene-editing-for-the-conservation-jxzdcpp27protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_33

Perspectives of Gene Editing for the Conservation of Plant Genetic Resources]]>Perspectives of Gene Editing for the Conservation of Plant Genetic ResourcesProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_33protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_332026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_33www.protocols.io/view/perspectives-of-gene-editing-for-the-conservation-jxzdcpp27www.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxzecpp3fprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_27

Long-Term Preservation by the Cryopreservation Method of Swietenia humilis Zucc]]>Long-Term Preservation by the Cryopreservation Method of Swietenia humilis ZuccProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_27protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_272026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_27www.protocols.io/view/long-term-preservation-by-the-cryopreservation-met-jxzecpp3fwww.protocols.io/view/application-of-nanomaterials-in-the-conservation-o-jxzfcpp3pprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_31

Application of Nanomaterials in the Conservation of Plant Genetic Resources]]>Application of Nanomaterials in the Conservation of Plant Genetic ResourcesProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_31protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_312026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_31www.protocols.io/view/application-of-nanomaterials-in-the-conservation-o-jxzfcpp3pwww.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxzgcpp3xprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_16

Medium-Term Preservation by the In Vitro Minimum Growth Method of Cordia alliodora (Ruiz and Pavón) Oken]]>Medium-Term Preservation by the In Vitro Minimum Growth Method of Cordia alliodora (Ruiz and Pavón) OkenProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_16protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_162026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_16www.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxzgcpp3xwww.protocols.io/view/long-term-preservation-by-cryopreservation-method-jxzhcpp37protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_26

Long-Term Preservation by Cryopreservation Method Gmelina arborea Roxb.]]>Long-Term Preservation by Cryopreservation Method Gmelina arborea Roxb.Protocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_26protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_262026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_26www.protocols.io/view/long-term-preservation-by-cryopreservation-method-jxzhcpp37www.protocols.io/view/long-term-preservation-by-cryopreservation-method-jxzicpp4fprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_28

Long-Term Preservation by Cryopreservation Method Swietenia macrophylla King]]>Long-Term Preservation by Cryopreservation Method Swietenia macrophylla KingProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_28protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_282026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_28www.protocols.io/view/long-term-preservation-by-cryopreservation-method-jxzicpp4fwww.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxzjcpp4pprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_14

Medium-Term Preservation by the In Vitro Minimum Growth Method of Cedrela odorata L.]]>Medium-Term Preservation by the In Vitro Minimum Growth Method of Cedrela odorata L.Protocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_14protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_142026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_14www.protocols.io/view/medium-term-preservation-by-the-in-vitro-minimum-g-jxzjcpp4pwww.protocols.io/view/in-vitro-propagation-of-em-limonium-cordatum-i-l-m-jxzkcpp4xprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.1007/978-1-0716-5130-8_9

In Vitro Propagation of Limonium cordatum (L.) Mill. for Short-Term Conservation]]>In Vitro Propagation of Limonium cordatum (L.) Mill. for Short-Term ConservationProtocols Importerdx.doi.org/10.1007/978-1-0716-5130-8_9protocols.io, Published online: 2026-04-16; | dx.doi.org/10.1007/978-1-0716-5130-8_92026-04-16protocols.iodx.doi.org/10.1007/978-1-0716-5130-8_9www.protocols.io/view/in-vitro-propagation-of-em-limonium-cordatum-i-l-m-jxzkcpp4xwww.protocols.io/view/deep-learning-identification-of-distinct-eeg-signa-jxzwcpp7fprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.6qpvrbnd2lmk/v1

Deep Learning Identification of Distinct EEG Signatures of Recent vs. Established Chronicity in Fibromyalgia]]>Deep Learning Identification of Distinct EEG Signatures of Recent vs. Established Chronicity in Fibromyalgiajean-marie amodeodx.doi.org/10.17504/protocols.io.6qpvrbnd2lmk/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.6qpvrbnd2lmk/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.6qpvrbnd2lmk/v1www.protocols.io/view/deep-learning-identification-of-distinct-eeg-signa-jxzwcpp7fwww.protocols.io/view/protocol-for-assessing-lysozyme-like-activity-in-g-jxzzcpp77protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.dm6gp7kw1gzp/v1

Protocol for Assessing Lysozyme-Like Activity in Galleria mellonella Hemolymph using EnzChek Kit]]>Protocol for Assessing Lysozyme-Like Activity in Galleria mellonella Hemolymph using EnzChek KitJuan Jose Quispe Harodx.doi.org/10.17504/protocols.io.dm6gp7kw1gzp/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.dm6gp7kw1gzp/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.dm6gp7kw1gzp/v1www.protocols.io/view/protocol-for-assessing-lysozyme-like-activity-in-g-jxzzcpp77www.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jx2jcpqcpprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v3

Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seq]]>Nuclei Prep from Frozen Mouse Brain Tissues with Optional Debris Removals Methods for single nuclei RNA-seqGreggory A A Perrydx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v3protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v32026-04-16protocols.iodx.doi.org/10.17504/protocols.io.8epv5ypp6l1b/v3www.protocols.io/view/nuclei-prep-from-frozen-mouse-brain-tissues-with-o-jx2jcpqcpwww.protocols.io/view/development-and-validation-of-a-multi-dimensional-jx2rcpqd7protocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.dm6gp7kb1gzp/v1

Development and Validation of a Multi-Dimensional Caregiver Taxonomy for Older Adult Caregivers: Study Protocol]]>Development and Validation of a Multi-Dimensional Caregiver Taxonomy for Older Adult Caregivers: Study ProtocolNabil Zarydx.doi.org/10.17504/protocols.io.dm6gp7kb1gzp/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.dm6gp7kb1gzp/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.dm6gp7kb1gzp/v1www.protocols.io/view/development-and-validation-of-a-multi-dimensional-jx2rcpqd7www.protocols.io/view/systematic-literature-review-protocol-the-impacts-jx25cpqg7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.j8nlkzjwwl5r/v1

Systematic Literature Review Protocol - The impacts of artificial intelligence on environmental sustainability and human well-being]]>Systematic Literature Review Protocol - The impacts of artificial intelligence on environmental sustainability and human well-beingnoemi.luna.carmenodx.doi.org/10.17504/protocols.io.j8nlkzjwwl5r/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.j8nlkzjwwl5r/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.j8nlkzjwwl5r/v1www.protocols.io/view/systematic-literature-review-protocol-the-impacts-jx25cpqg7www.protocols.io/view/fabrication-of-pdms-microfluidic-chip-device-jx29cpqh7protocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.j8nlkzjwdl5r/v1

FABRICATION OF PDMS MICROFLUIDIC CHIP DEVICE]]>FABRICATION OF PDMS MICROFLUIDIC CHIP DEVICENevena Milivojević Dimitrijevićdx.doi.org/10.17504/protocols.io.j8nlkzjwdl5r/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.j8nlkzjwdl5r/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.j8nlkzjwdl5r/v1www.protocols.io/view/fabrication-of-pdms-microfluidic-chip-device-jx29cpqh7www.protocols.io/view/localization-of-psyn-aggregates-to-either-axons-or-jx3mcpqk7protocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.dm6gp7kjpgzp/v1

Localization of pSYN aggregates to either axons or dendrites]]>Localization of pSYN aggregates to either axons or dendritesAndrew D Sauerbeckdx.doi.org/10.17504/protocols.io.dm6gp7kjpgzp/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.dm6gp7kjpgzp/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.dm6gp7kjpgzp/v1www.protocols.io/view/localization-of-psyn-aggregates-to-either-axons-or-jx3mcpqk7www.protocols.io/view/bidmc-tmc-assessing-rna-integrity-from-ffpe-sample-jx3ncpqmfprotocols.io, Published online: 2026-04-16; doi:dx.doi.org/10.17504/protocols.io.e6nvwwyjwvmk/v1

BIDMC-TMC Assessing RNA integrity from FFPE samples for spatial transcriptomics]]>BIDMC-TMC Assessing RNA integrity from FFPE samples for spatial transcriptomicsShuoshuo Wangdx.doi.org/10.17504/protocols.io.e6nvwwyjwvmk/v1protocols.io, Published online: 2026-04-16; | dx.doi.org/10.17504/protocols.io.e6nvwwyjwvmk/v12026-04-16protocols.iodx.doi.org/10.17504/protocols.io.e6nvwwyjwvmk/v1www.protocols.io/view/bidmc-tmc-assessing-rna-integrity-from-ffpe-sample-jx3ncpqmfwww.protocols.io/view/fabrication-of-an-acrylic-microfluidic-chip-system-jx3pcpqmpprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.n2bvjkw85gk5/v1

Fabrication of an acrylic microfluidic chip system]]>Fabrication of an acrylic microfluidic chip systemNevena Milivojević Dimitrijevićdx.doi.org/10.17504/protocols.io.n2bvjkw85gk5/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.n2bvjkw85gk5/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.n2bvjkw85gk5/v1www.protocols.io/view/fabrication-of-an-acrylic-microfluidic-chip-system-jx3pcpqmpwww.protocols.io/view/rnaseq-protocol-at-plasmidsaurus-jx3vcpqn7protocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1

RNASEQ protocol at Plasmidsaurus]]>RNASEQ protocol at PlasmidsaurusBeth Martindx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.ewov1rwqolr2/v1www.protocols.io/view/rnaseq-protocol-at-plasmidsaurus-jx3vcpqn7www.protocols.io/view/mpra-library-design-and-cloning-of-functional-evol-jx32cpqqfprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.rm7vz4dx5lx1/v1

MPRA library design and cloning of functional evolution, modification, and derivatization of mammalian developmental enhancers]]>MPRA library design and cloning of functional evolution, modification, and derivatization of mammalian developmental enhancersTony Lidx.doi.org/10.17504/protocols.io.rm7vz4dx5lx1/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.rm7vz4dx5lx1/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.rm7vz4dx5lx1/v1www.protocols.io/view/mpra-library-design-and-cloning-of-functional-evol-jx32cpqqfwww.protocols.io/view/bcrseq-analysis-using-dandelion-jx4pcpqvpprotocols.io, Published online: 2026-04-17; doi:dx.doi.org/10.17504/protocols.io.eq2lyorwwgx9/v1

BCRseq Analysis using Dandelion]]>BCRseq Analysis using DandelionOliver Skinnerdx.doi.org/10.17504/protocols.io.eq2lyorwwgx9/v1protocols.io, Published online: 2026-04-17; | dx.doi.org/10.17504/protocols.io.eq2lyorwwgx9/v12026-04-17protocols.iodx.doi.org/10.17504/protocols.io.eq2lyorwwgx9/v1www.protocols.io/view/bcrseq-analysis-using-dandelion-jx4pcpqvpwww.protocols.io/view/fetal-brain-tissue-dissociation-protocol-for-singl-jx5tcpq6pprotocols.io, Published online: 2026-04-18; doi:dx.doi.org/10.17504/protocols.io.n92ld4xrxl5b/v1

Fetal Brain Tissue Dissociation Protocol for Single-Cell RNA Sequencing]]>Fetal Brain Tissue Dissociation Protocol for Single-Cell RNA SequencingSiddharth Singhdx.doi.org/10.17504/protocols.io.n92ld4xrxl5b/v1protocols.io, Published online: 2026-04-18; | dx.doi.org/10.17504/protocols.io.n92ld4xrxl5b/v12026-04-18protocols.iodx.doi.org/10.17504/protocols.io.n92ld4xrxl5b/v1www.protocols.io/view/fetal-brain-tissue-dissociation-protocol-for-singl-jx5tcpq6p